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What does GCK stand for?

Provided by the Springer Nature SharedIt content-sharing initiative Glucokinase (GCK) regulates insulin secretion to maintain appropriate blood glucose levels.

Is GCK a static enzyme?

GCK is a dynamic 52-kDa enzyme consisting of 465 amino acid residues, which fold into a large and a small domain. Between the two domains is a cleft forming the active site where glucose binds. The orientation of the two domains is not static, as GCK exists in multiple conformational ensembles [18, 19].

Which GCK variant grows faster than wt?

The hyperactive variant (p.W99R) grew faster than WT, while the four disease-associated variants with reduced activity (p.F150S, p.R191W, p.G223S and p.G261R) grew slower than WT (Fig. 1 B). The growth of the eight GCK variants was consistent with their level of activity, showing that the complementation assay could assess GCK activity.

How are variants of GCK identified?

Variants in GCK were identified using sequencing. Samples were collected from a population-based cohort of 6,058 individuals both with and without diabetes , 2,930 patients with newly-diagnosed diabetes , patients diagnosed with GCK -MODY and from a population of 1,146 Danish children .